Search For separation of low molecular weight proteins, use Novex Tricine gels which provide increased resolution of proteins with molecular weights as low as 2.5 kDa. These protein standards and ladders are consistent from lot to lot and are strictly quality controlled on appropriate gels to help ensure consistent band migration and intensity. Accelerating generation of single cell clones by using CellRaft AIR System coupled with Cellular assay using brightfield and fluorescence-based live cell imaging. Separation of native proteins, neutral pH for high sensitivity applications, Separation of proteins based on isoelectric point, Separation of proteins based on size and activity, High-throughput gel system, that eliminates the need for buffers, SureCast reagents, other popular polyacrylamide reagents, Western blot transfer and analysis, and all techniques in which protein integrity is crucial, Mini with larger well capacity (WedgeWell, up to 60 L), Broad-range separation of proteins using traditional Laemmli-style gels, Separation of small proteins and peptides, High throughput for recombination product analysis and protein profiling, Leak-free designminimize the number of failed gels, waste less time, Unique tilt featureminimize spillage and confidently load acrylamide solutions, Simple component assemblyusing a single-motion load-and-lock mechanism, Ready-to-pour cassettes, casting stand not required, cassettes sealed on three sides (no gel leakage), Special coating provides uniform polymerization rate for a more consistent handcast gel, High capacity sample wellsload up to 60 L per well. The choice of whether to use one chemistry or another depends on the abundance of the protein youre separating, the size of the protein, and your downstream application. HlUK#9)V >B:xo&Urt
8mqJO. Read more Spectra Multicolor High Range Protein Ladder is a mixture of eight (8) blue-, green-and orange-stained proteins (40 to 300kDa) for use as size standards for high-MW proteins in gel electrophoresis and Western blotting. 0000002299 00000 n
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For separation of proteins by isoelectroctric point (pI), use Novex IEF gels. 0000096617 00000 n
Not for use in diagnostic procedures. 78k w7 Not for use in diagnostic procedures. The ladder is ready to use without heating and diluting. 0000005895 00000 n
For Research Use Only. It's that simple. Search 0000109261 00000 n
Search Low molecular weight proteins, western blotting, in-gel staining, and denaturing applications. Create Account, Protein Gel Electrophoresis and Western Blotting Education Center, Spectroscopy, Elemental & Isotope Analysis, Preclinical to Companion Diagnostic Development, Microbiological Media and Media Additives, Gel Electrophoresis Equipment and Supplies, Protein Isolation and Purification Information, Western Blot Antibody Dilution Calculator, Protein Modification and Crosslinking Information. The ready-to-use Thermo Scientific PageRuler NIR Protein Ladder contains a mixture of 10 proteins ranging from 11K to 250K. 0000096644 00000 n
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View upcoming and recorded webinars for in-depth understanding of molecular biology techniques and resources. Thermo Fisher Scientific. Don't have an account ? Don't have an account ? 0000096054 00000 n
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Find our E-Gel selection table that will guide you in choosing the best products for your experiments. Designed to give you total felxibility in your application. 0000008463 00000 n
Get started with our money-saving Protein Gels Welcome Packs, Mini Gels: Mini Gel Tank or XCell SureLock Mini-Cell, Midi Gels: XCell4 SureLock Midi-Cell or Bio-Rad Criterion (with adapters only). Separated proteins and nucleic acids in the gel can be stained or probed with fluorescent markers and then imaged and detected using gel documentation instruments, transilluminators, densitometers and scanners. Create Account, 26616, 26619, 26634, 26625, 26628, LC5800, LC5699, LC5615, LC5925, LC5625, 10748010, 26630, 26632, 26614, 26637, LC5677, 26610, LC5801, 10747012, LC5688, LC5615, LC5602, 84785, 84786, LC5615, 26635, LC5928, 3921201, LC0725, LC5700, LC5606, P33350, C21852, Spectroscopy, Elemental & Isotope Analysis, Preclinical to Companion Diagnostic Development, Microbiological Media and Media Additives, Gel Electrophoresis Equipment and Supplies, Protein Standards and Ladders Selection Guide, Protein Electrophoresis Buffers and Reagents, Protein Gel Electrophoresis Chamber Systems, Spectra Multicolor Broad Range Protein Ladder, Spectra Multicolor High Range Protein Ladder, Spectra Multicolor Low Range Protein Ladder, SuperSignal Molecular Weight Protein Ladder, SuperSignal Enhanced Molecular Weight Protein Ladder, PageRuler Unstained High Range Protein Ladder, PageRuler Unstained Low Range Protein Ladder, PageRuler Unstained Broad Range Protein Ladder, Peppermint Stick Phosphoprotein Molecular Weight Standards, CandyCane Glycoprotein Molecular Weight Standards, Protein gel electrophoresis technical handbook, Protocols, tips & tricks, and electrophoresis troubleshooting, ~180, 130, 100, 70, 55, 40, 35, 25, 15, 10 kDa, ~250, 130, 100, 70, 55, 35, 25, 15, 10 kDa, ~260, 140, 100, 70, 50, 40, 35, 25, 15, 10 kDa, ~180, 115, 82, 64, 49, 37, 26, 19, 15, 6 kDa, ~260, 160, 110, 80, 60, 50, 40, 30, 20, 15, 10, 3.5 kDa, ~198, 98, 62, 49, 38, 28, 17, 14, 6, 3 kDa, Colorimetric, NIR fluorescence (700nm channel, blue bands), RGB fluorescence (550nm channel, orange bands), 5 L for electrophoresis, 5 L for blotting, 10 L for electrophoresis, 10 L for blotting, 10 L for electrophoresis, 5 L for blotting, 10 L for electrophoresis, 3 L for blotting, ~ 460, 268, 238, 171, 117, 71, 55, 41, 31 kDa, Colorimetric, NIR fluorescence (700 nm channel, blue bands), RGB fluorescence (550 nm channel, orange bands), ~250, 130, 95, 80, 70, 55, 43, 34, 30, 26, 15, 11 kDa, ~220, 120, 100, 80, 60, 50, 40, 30, 20 kDa, ~250, 130, 95, 70, 55, 43, 34, 26, 15, 11 kDa, IgG binding sites on 2 bands (80 and 30 kDa), 10 fluor-labeled, blue-stained bands, IgG binding sites on all bands, recommended for mouse monoclonal antibodies or weak binding antibody systems, Colorimetric, NIR fluorescence (700 nm channel), user defined detection system (2 bands), User defined detection system (not recommended for RGB fluorescence systems), Colorimetric, NIR fluorescence (670 nm red laser or 700 nm channels), ~500, 290, 240, 160, 116, 97, 66, 55, 40 kDa, ~460, 268, 238, 171, 117, 71, 55, 41, 31 kDa, ~200, 150, 120, 100, 85, 70, 60, 50, 40, 30, 25, 20, 15, 10 kDa, ~200, 116.3, 97.4, 66.3, 55.4, 36.5, 31, 21.5, 14.4, 6, 3.5, 2.5 kDa, ~250, 150, 100, 70, 50, 40, 30, 20, 15, 10, 5 kDa, ~220, 160, 120, 100, 90, 80, 70, 60, 50, 40, 30, 25, 20, 15, 10 kDa, ~10.7, 9.5, 8.3, 8.0, 7.8, 7.4, 6.9, 6.0, 5.3, 5.2, 4.5, 4.2, 3.5, ~1236, 1048, 720, 480, 242, 146, 66, 20 kDa, ~160, 120, 80, 60, 50, 40, 30, 20, 15, 10 kDa, Each protein standard contains a 6X His-tag enabling the detection with the InVision His-tag In-gel Stain, Colorimetric or methods that detect phosphorylated proteins such as Pro-Q Diamond phosphoprotein gel stains, Colorimetric or methods that detect glycosylated proteins such as Pro-Q Glycoprotein stain kits, 5 L for electrophoresis, 2.55 L for blotting. Choose from precast polyacrylamide gel electrophoresis (PAGE) chemistries designed for specific applications including broad range, high, or low molecular weight separations, SDS-PAGE, native PAGE, or IEF. Mini gels and wider, higher throughput, midi gels are offered in a variety of well and cassette formats. Read more NuPAGE Bis-Tris gels are precast polyacrylamide high-performance gels that offer optimized chemistry for denaturing gel applications. The adjustable vertical system gives researchers the flexibility of running five different path length gels. There are no gels to pour, buffers to make, or staining neededfor ultimate convenience. Clinical testing: How reliable and inventory-friendly methods are helping overcome post-pandemic BioGX launches CE-IVD marked multi-gene COVID-19 point-of-care test, CellaVision launches a complete workflow solution for low-volume hematology laboratories, 5 upcoming webinars to fuel your research goals, GC troubleshooting tips and tricks from inlet through to detection, Managing the risk of nitrosamines in medicinal drugs. Load up to 60 L per well with Bolt gels. Watch fun and educational videos on molecular biology techniques, how-tos, and tips and tricks. Invitrogen iBlot 2 Transfer Stacks, nitrocellulose, Invitrogen SeeBlue Plus2 Pre-stained Protein Standard, Invitrogen Novex Tris-Glycine Mini Gels, WedgeWell format, Thermo Scientific Pierce Silver Stain Kit, Vybrant DyeCycle Green and Orange Stains, Invitrogen NuPAGE Bis-Tris Protein Gels, Thermo Scientific Owl EasyCast B2 Mini Gel Electrophoresis Systems, Classic Series 96 Well Compatible Gel Systems, Thermo Scientific Bond-Breaker TCEP Solution, Neutral pH, Thermo Scientific Spectra Multicolor High Range Protein Ladder, Thermo Scientific Spectra Multicolor Broad Range Protein Ladder, Invitrogen NativeMark Unstained Protein Standard, Thermo Scientific PageRuler Plus Prestained Protein Ladder, 10 to 250 kDa, Invitrogen MagicMark XP Western Protein Standard, Corporate Social Responsibility Statement. Protein gels performance guaranteeInvitrogen precast protein gels are designed and tested to deliver superior performance, reliability, reproducibility, and consistency of quality. 0000093852 00000 n
With the built-in timer, you can challenge your friends and labmates to solve it faster than you. Search Thermo Fisher Scientific. We create value for our key stakeholders through two premier brands, Thermo Scientific and Fisher Scientific, which offer a unique combination of continuous technology development and the most convenient purchasing options. Learn how the right protein gel chemistry can help you achieve better protein separation and western blots. Use No-Stain Protein Labeling Reagent for total protein normalization to help improve the accuracy of your quantitative western blotting data. 0000144246 00000 n
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Explore our protein gel options. Or select a system for pouring and casting your own gels. The Thermo Scientific PageRuler Unstained High Range Protein Ladder is a mix of eight purified recombinant proteins ranging from 60K to 250K. 0000143998 00000 n
The 175 Adjustable Vertical Gel Systemis not available in the US or Canada. This helps reduce protein modifications and helps produce sharp band resolution. 0000109525 00000 n
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Electrophoresis equipment is used to separate mixtures of protein, DNA or RNA, based on their electric charge, size and other physical characteristics, by passing them through a medium such as a polyacrylamide gel, an agarose gel or a capillary tube. Not for use in diagnostic procedures. 0000002442 00000 n
Fast 2035 minute run times. 0000094292 00000 n
For denaturing gel electrophoresis or SDS PAGE there are many options available based on the application, and size of the protein of interest. Optimized for analysis of a wide range of protein targets, Prestained, unstained and western blot ladders, Stains for every application from quick visualization to high-sensitivity applications, Quality transfers using less volume of methanol-based transfer buffers, Mini, midi gel electrophoresis, and transfers. For separation of proteins under nondenaturing conditions or native page, three gel systems are recommended- NativePAGE Bis-Tris, Novex Tris-Glycine and NuPAGE Tris-Acetate. For separation of a broad range of proteins, two chemistries, Bis-Tris and Tris-glycine, have been optimized for performance and long shelf life. Read more Novex Tris-Glycine Mini Gels, WedgeWell format, are polyacrylamide gels based on traditional Laemmli protein electrophoresis, offering reproducible separation of a wide range of proteins into well-resolved band. This webinar will highlight the different protein modifications occurring during electrophoresis, the importance of pH in sample integrity, how to choose the right gel chemistry for specific research applications, and how to introduce the right new gel chemistry in your research workflow. Both models are perfect for DNA/RNA submarine electrophoresis. Create Account. Our portfolio of high-quality protein electrophoresis products unites gels, gel tanks, protein gel handcast system, stains, molecular weight markers and standards, running buffers, and blotting products for your protein analysis experiments. Precast gels with a gradient can be used or gels can be hand cast. All prestained protein ladders are compatible for use in western blotting. Electrophoresis equipment includes horizontal and vertical gel electrophoresis chambers, isoelectric focusing systems, 2D electrophoresis and capillary electrophoresis instruments. U$2@1"k6)O ]+~^lZ?6X wF
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The NativeMark Unstained Protein Standard is a ready-to-use protein standard designed for molecular weight estimation of proteins using native gel electrophoresis. We stand behind the performance of our protein gels. Our mission is to enable our customers to make the world healthier, cleaner and safer. Choose from a variety of protein ladders (molecular weight markers) for protein electrophoresis and western blotting applications. 0000002993 00000 n
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'%!f)%zk&=7s6S46!f[r>|T5,=NIJIAGcW[~uLVP] l99Dz%kJ&Y/IG.$l1,K)Z9T?@`23@b%@N` vi$OX$-=y6 qQBtg.$PZR^;W 8#T9?3Th_We Bis-Tris chemistry provides a neutral (pH 7.0) environment during electrophoresis, which may result in better sample integrity and stability of the gel. ROCKFORD, Ill. (January 16, 2012) Thermo Fisher Scientific, the world leader in serving science, today announced the addition of two new protein molecular weight markers for determining the size of proteins in gel electrophoresis and Western blotting applications. However, gaining publication quality results straightaway is not exactly the norm when performing western blotting.